SNX16 Antibody

• Catalog Number: orb1246933
• Category: Antibodies
• Description: SNX16 Antibody
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, IHC, WB
• Predicted Reactivity: Bovine, Canine, Mouse, Porcine, Rat
• Reactivity: Human
• Immunogen: The immunogen for this antibody is: ATPYVPVPMPIGN-C
• Concentration: 500 ug/mL
• Dilution range: Peptide ELISA: antibody detection limit dilution 1:64000.Western Blot:Approx 38kDa band observed in lysates of cell line Daudi (calculated MW of 39.2kDa according to NP_071416.2). In transfected HEK293 transiently expressing SNX16 a band of approx. 40kDa is observed. This band is not observed in the non-transfected HEK293. Recommended concentration: 0.05-0.2ug/ml.Immunohistochemistry:Paraffin embedded Human Placenta, Tonsil and Breast. Recommended concentration: 3.75ug/ml.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• Target: SNX16
• UniProt ID: P57768
• NCBI: NP_690050.1, NP_071416.2, NP_690049.1
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: SNX16, sorting nexin 16, DKFZp666H147
• Note: For research use only
• Application notes: Peptide ELISA: antibody detection limit dilution 1:64000.Western Blot:Approx 38kDa band observed in lysates of cell line Daudi (calculated MW of 39.2kDa according to NP_071416.2). In transfected HEK293 transiently expressing SNX16 a band of approx. 40kDa is observed. This band is not observed in the non-transfected HEK293. Recommended concentration: 0.05-0.2ug/ml.Immunohistochemistry:Paraffin embedded Human Placenta, Tonsil and Breast. Recommended concentration: 3.75ug/ml.
• Expiration Date: 12 months from date of receipt.

orb1246933 (0.05 ug/ml) staining of Daudi lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

HEK293 overexpressing SNX16 and probed with orb1246933 (mock transfection in first lane).

orb1246933 (3.75 ug/ml) staining of paraffin embedded Human Placenta. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

orb1246933 (3.75 ug/ml) staining of paraffin embedded Human Tonsil. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

orb1246933 (3.75 ug/ml) staining of paraffin embedded Human Breast. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.