SNW1 Antibody / NCoA-62 / SKIP

SKU: orb1825523

Description

Nuclear receptor coactivator NCOA62/SNW1 (also called Nuclear Protein SkiP, SKIIP, Ski-interacting protein) is a member of the SNW gene family, encodes a coactivator that enhances transcription from some Pol II promoters. This coactivator can bind to the ligand-binding domain of the vitamin D receptor and to retinoid receptors to enhance vitamin D-, retinoic acid-, estrogen-, and glucocorticoid-mediated gene expression. It can also interact with poly(A)-binding protein 2 to directly control the expression of muscle-specific genes at the transcriptional level. Finally, the protein may be involved in oncogenesis since it interacts with a region of SKI oncoproteins that is required for transforming activity.

Images & Validation

• Tested Applications: FACS, IF
• Dilution range: Flow cytometry: 1-2ug/million cells,Immunofluorescence: 2-4ug/ml
• Reactivity: Human

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: Mouse IgG2a
• Clone No.: PCRP-SNW1-1C12
• Immunogen: A recombinant fragment of human SNW1 protein was used as the immunogen for the SNW1 antibody.

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Disclaimer: For research use only

Immunofluorescent staining of PFA-fixed human HeLa cells with SNW1 antibody (clone PCRP-SNW1-1C12) followed by goat anti-mouse IgG-CF488; Membrane stained with phalloidin (red).

Flow cytometry testing of PFA-fixed human HeLa cells with SNW1 antibody (clone PCRP-SNW1-1C12) followed by goat anti-mouse IgG-CF488 (blue), Red = unstained cells.

SDS-PAGE analysis of purified, BSA-free SNW1 antibody (clone PCRP-SNW1-1C12) as confirmation of integrity and purity.

Analysis of a HuProt (TM) microarray containing more than 19000 full-length human proteins using SNW1 antibody (clone PCRP-SNW1-1C12). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProt (TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProt (TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a mAb to its intended target. A mAb is considered to specific to its intended target, if the mAb has an S-score of at least 2.5. For example, if a mAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that mAb to protein X is equal to 29.

Quick Database Links

UniProt

Q13573