SNRPA Antibody

• Catalog Number: orb1257030
• Category: Antibodies
• Description: SNRPA Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, IHC, WB
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-282 of human SNRPA (NP_004587.1).
• Concentration: batch dependent
• Dilution range: WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: Observed: 31kDa
• Target: SNRPA
• UniProt ID: P09012
• Storage: Store at -20°C. Avoid freeze / thaw cycles.
• Buffer/Preservatives: PBS with 0.02% sodium azide, 50% glycerol, pH 7.3.
• Alternative names: SNRPA, U1A, Mud1, U1-A
• Note: For research use only
• Application notes: WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200
• Expiration Date: 12 months from date of receipt.

Western blot analysis of extracts of various cell lines, using SNRPA antibody (orb1257030) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Immunohistochemistry of paraffin-embedded rat liver using SNRPA Antibody (orb1257030) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded rat brain using SNRPA Antibody (orb1257030) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded rat heart using SNRPA Antibody (orb1257030) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded human breast using SNRPA Antibody (orb1257030) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded human stomach using SNRPA Antibody (orb1257030) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded human gastric cancer using SNRPA Antibody (orb1257030) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded human leiomyoma of uterus using SNRPA Antibody (orb1257030) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded human adenomyosis using SNRPA Antibody (orb1257030) at dilution of 1:100 (40x lens).

Chromatin immunoprecipitation analysis of extracts of 293T cells, using TriMethyl-Histone H3-K27 antibody (orb1258185) and rabbit IgG. P1 and P2 were located on ANO2 gene. The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.

Immunohistochemistry of paraffin-embedded mouse heart using SNRPA Antibody (orb1257030) at dilution of 1:100 (40x lens).

Immunofluorescence analysis of HeLa cells using SNRPA antibody (orb1257030).