SNRPA Antibody

SKU: orb1257030

Description

SNRPA Antibody

Images & Validation

• Tested Applications: IF, IHC, WB
• Reactivity: Human, Mouse, Rat
• Application Notes:
  WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-282 of human SNRPA (NP_004587.1).
• Target: SNRPA
• Molecular Weight: Observed: 31kDa
• Purification: Affinity purification
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Liquid
• Concentration: batch dependent
• Disclaimer: For research use only

Alternative Names

SNRPA, U1A, Mud1, U1-A

Western blot analysis of extracts of various cell lines, using SNRPA antibody (orb1257030) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Immunohistochemistry of paraffin-embedded rat liver using SNRPA Antibody (orb1257030) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded rat brain using SNRPA Antibody (orb1257030) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded rat heart using SNRPA Antibody (orb1257030) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded human breast using SNRPA Antibody (orb1257030) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded human stomach using SNRPA Antibody (orb1257030) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded human gastric cancer using SNRPA Antibody (orb1257030) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded human leiomyoma of uterus using SNRPA Antibody (orb1257030) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded human adenomyosis using SNRPA Antibody (orb1257030) at dilution of 1:100 (40x lens).

Chromatin immunoprecipitation analysis of extracts of 293T cells, using TriMethyl-Histone H3-K27 antibody (orb1258185) and rabbit IgG. P1 and P2 were located on ANO2 gene. The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.

Immunohistochemistry of paraffin-embedded mouse heart using SNRPA Antibody (orb1257030) at dilution of 1:100 (40x lens).

Immunofluorescence analysis of HeLa cells using SNRPA antibody (orb1257030).

Quick Database Links

Gene Symbol

SNRPA

UniProt

P09012