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Catalog Number | orb1248633 |
---|---|
Category | Antibodies |
Description | SNCA Antibody |
Species/Host | Goat |
Clonality | Polyclonal |
Tested applications | ELISA, IHC, WB |
Predicted Reactivity | Canine, Porcine, Rat |
Reactivity | Human, Mouse |
Immunogen | The immunogen for this antibody is: C-ATGFVKKDQLGK |
Concentration | 500 ug/mL |
Dilution range | Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 17kDa band observed in Human Brain (Amygdala) and fetal Mouse Brain lysates (calculated MW of 14.5kDa according to Human NP_000336.1 and Mouse NP_033247.1). The observed molecular weight corresponds to earlier findings with different antibodies from other commercial sources. Recommended concentration: 0.1-0.3ug/ml. Primary incubation was 1 hour.Immunohistochemistry:Paraffin embedded Human Brain (Cerebellum). Recommended concentration: 5ug/ml. |
Form/Appearance | Liquid |
Conjugation | Unconjugated |
MW | Approx 17 kDa |
Target | SNCA |
UniProt ID | P37840 |
NCBI | NP_009292.1, NP_000336.1 |
Storage | Aliquot and store at -20°C. Minimize freezing and thawing. |
Buffer/Preservatives | Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing. |
Alternative names | SNCA, synuclein, alpha (non A4 component of amyloi Read more... |
Note | For research use only |
Application notes | Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 17kDa band observed in Human Brain (Amygdala) and fetal Mouse Brain lysates (calculated MW of 14.5kDa according to Human NP_000336.1 and Mouse NP_033247.1). The observed molecular weight corresponds to earlier findings with different antibodies from other commercial sources. Recommended concentration: 0.1-0.3ug/ml. Primary incubation was 1 hour.Immunohistochemistry:Paraffin embedded Human Brain (Cerebellum). Recommended concentration: 5ug/ml. |
Expiration Date | 12 months from date of receipt. |
orb1248633 (0.1 ug/ml) staining of Human Amygdala (A) and fetal Mouse (B) Brain lysate (35 ug protein in RIPA buffer). Detected by chemiluminescence.
orb1248633 (5 ug/ml) staining of paraffin embedded Human Cerebellum. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
ELISA, ICC, IF, IHC-Fr, IHC-P, WB | |
Bovine, Gallus, Porcine | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
IHC-P, WB | |
Human, Mouse, Rat | |
Mouse | |
Monoclonal | |
Unconjugated |
ELISA, IF, IHC-P, WB | |
Bovine, Porcine | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
IF, IH, WB | |
Human, Mouse, Primate, Rat, Sheep | |
Rabbit | |
Polyclonal | |
Unconjugated |
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