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SNCA Antibody

Catalog Number: orb1248633

DispatchUsually dispatched within 5-10 working days
$ 813.00
Catalog Numberorb1248633
CategoryAntibodies
DescriptionSNCA Antibody
Species/HostGoat
ClonalityPolyclonal
Tested applicationsELISA, IHC, WB
Predicted ReactivityCanine, Porcine, Rat
ReactivityHuman, Mouse
ImmunogenThe immunogen for this antibody is: C-ATGFVKKDQLGK
Concentration500 ug/mL
Dilution rangePeptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 17kDa band observed in Human Brain (Amygdala) and fetal Mouse Brain lysates (calculated MW of 14.5kDa according to Human NP_000336.1 and Mouse NP_033247.1). The observed molecular weight corresponds to earlier findings with different antibodies from other commercial sources. Recommended concentration: 0.1-0.3ug/ml. Primary incubation was 1 hour.Immunohistochemistry:Paraffin embedded Human Brain (Cerebellum). Recommended concentration: 5ug/ml.
Form/AppearanceLiquid
ConjugationUnconjugated
MWApprox 17 kDa
TargetSNCA
UniProt IDP37840
NCBINP_009292.1, NP_000336.1
StorageAliquot and store at -20°C. Minimize freezing and thawing.
Buffer/PreservativesSupplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Alternative namesSNCA, synuclein, alpha (non A4 component of amyloi
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NoteFor research use only
Application notesPeptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 17kDa band observed in Human Brain (Amygdala) and fetal Mouse Brain lysates (calculated MW of 14.5kDa according to Human NP_000336.1 and Mouse NP_033247.1). The observed molecular weight corresponds to earlier findings with different antibodies from other commercial sources. Recommended concentration: 0.1-0.3ug/ml. Primary incubation was 1 hour.Immunohistochemistry:Paraffin embedded Human Brain (Cerebellum). Recommended concentration: 5ug/ml.
Expiration Date12 months from date of receipt.
SNCA Antibody

orb1248633 (0.1 ug/ml) staining of Human Amygdala (A) and fetal Mouse (B) Brain lysate (35 ug protein in RIPA buffer). Detected by chemiluminescence.

SNCA Antibody

orb1248633 (5 ug/ml) staining of paraffin embedded Human Cerebellum. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

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