SNCA Antibody

• Catalog Number: orb1248632
• Category: Antibodies
• Description: SNCA Antibody
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, IHC, WB
• Predicted Reactivity: Porcine
• Reactivity: Human
• Immunogen: The immunogen for this antibody is: C-KDQLGKNEEGAPQE
• Concentration: 500 ug/mL
• Dilution range: Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 17kDa band observed in Human Brain (Amygdala) lysates (calculated MW of 14.5kDa according to NP_000336.1). The observed molecular weight corresponds to earlier findings with different antibodies from other commercial sources. Recommended concentration: 0.1-0.3ug/ml. Primary incubation was 1 hour.Immunohistochemistry:Paraffin embedded Human Brain (Cerebellum). Recommended concentration: 5ug/ml.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: Approx 17 kDa
• Target: SNCA
• UniProt ID: P37840
• NCBI: NP_000336.1
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: SNCA, synuclein, alpha (non A4 component of amyloi
• Note: For research use only
• Application notes: Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 17kDa band observed in Human Brain (Amygdala) lysates (calculated MW of 14.5kDa according to NP_000336.1). The observed molecular weight corresponds to earlier findings with different antibodies from other commercial sources. Recommended concentration: 0.1-0.3ug/ml. Primary incubation was 1 hour.Immunohistochemistry:Paraffin embedded Human Brain (Cerebellum). Recommended concentration: 5ug/ml.
• Expiration Date: 12 months from date of receipt.

orb1248632 (0.1 ug/ml) staining of Human Amygdala lysate (35 ug protein in RIPA buffer). Detected by chemiluminescence.

orb1248632 (5 ug/ml) staining of paraffin embedded Human Cerebellum. Steamed antigen retrieval with citrate buffer Ph 6, AP-staining.