SMYD4 Antibody

• Catalog Number: orb1256472
• Category: Antibodies
• Description: SMYD4 Antibody
• Target: SMYD4
• Clonality: Polyclonal
• Isotype: IgG
• Conjugation: Unconjugated
• Reactivity: Human, Mouse, Rat
• Form/Appearance: Liquid
• Concentration: batch dependent
• Buffer/Preservatives: Liquid
• Purification: Affinity purification
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-260 of human SMYD4 (NP_443160.2).
• UniProt ID: Q8IYR2
• MW: Observed: 100kDa
• Tested applications: IF, IHC, WB
• Application notes: WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:100
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: SET and MYND domain-containing protein 4, 211-, SM
• Research Area: Cancer Biology
• Note: For research use only

Western blot analysis of extracts of various cell lines, using SMYD4 antibody (orb1256472) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

Immunohistochemistry of paraffin-embedded human gastric cancer using SMYD4 antibody (orb1256472) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded mouse jejunum using SMYD4 antibody (orb1256472) at dilution of 1:100 (40x lens).

Immunofluorescence analysis of A-549 cells using SMYD4 antibody (orb1256472). Blue: DAPI for nuclear staining.