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Smoothened Agonist (SAG)

SKU: orb1223671

Description

SAG is a potent Smoothened (Smo) receptor agonist (Kd = 59 nM); potently activates the Hedgehog signaling pathway in Shh-light 2 cells (EC50 ~ 3 nM).

Research Area

Stem Cell & Developmental Biology; Pharmacology & Drug Discovery

Images & Validation

Key Properties

CAS Number912545-86-9
MW490.06
Purity>98% (HPLC)
FormulaC28H28ClN3OS
SMILESCNC1CCC(CC1)N(CC1=CC=CC(=C1)C1=CC=NC=C1)C(=O)C1=C(Cl)C2=CC=CC=C2S1
TargetHedgehog (Hh)
SolubilityEthanol: 40 mg/mL warmed (75.97 mM); Water: 100 mg/mL (189.92 mM); DMSO: 71 mg/mL warmed (134.84 mM)

Bioactivity

In Vivo
Animal model: Pregnant C57BL/6J mice. Dosage: 15, 17, 20 mg/kg. Administration: A single i.p. Result: Effective in ca. 80% of the embryos and increased Gli1 and Gli2 mRNA expression in the limb bud, with Gli1 mRNA being the most upregulated at the dose of 20 mg/kg.

Storage & Handling

StorageStorage temperature: -20°C. Stability: ≥ 2 years
Expiration Date12 months from date of receipt.
DisclaimerFor research use only

Alternative Names

Smoothened Agonist

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Protocol Information

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500 mg
5 mg
$ 170.00
10 mg
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25 mg
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50 mg
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100 mg
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200 mg
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