Hh-Ag1.5

SKU: orb1702838

Description

Hh-Ag1.5 is a potent small molecule agonist of the Hedgehog pathway and Smoothened receptor (EC50: 1 nM). It is used in research to study stem cell biology, enabling the differentiation of hiPSCs into neural and skin lineages in vitro and demonstrating hepatoprotective effects in models of liver failure.

Research Area

Pharmacology & Drug Discovery, Stem Cell & Developmental Biology

Key Properties

• CAS Number: 612542-14-0
• MW: 526.04
• Purity: 99.87% (May vary between batches)
• Formula: C₂₈H₂₆ClF₂N₃OS
• SMILES: CN[C@H]1CC[C@@H](CC1)N(Cc1cccc(c1)-c1ccncc1)C(=O)c1sc2c(F)ccc(F)c2c1Cl
• Target: Hedgehog/Smoothened
• Solubility: DMSO:26.3 mg/mL (50 mM);10% DMSO+40% PEG300+5% Tween 80+45% Saline:2 mg/mL (3.8 mM)

Bioactivity

• Target IC50:
  Hedgehog:1 nM (EC50)
• In Vitro:
  Hh-Ag1.5 completely binds to the Smo-containing membranes with the Ki value of 0.52 nM. The Smo agonist Hh-Ag1.5 displays nanomolar-range potency stimulation of Gli-responsive reporter activity, with an EC50 of 7 nM. Hh-Ag1.5 (5 μM; 3 weeks) facilitates the dramatic expansion of quiescent CD133+ CD45- liver stem cells (Hh3A cells) enriched from the non-injured liver in vitro.

Storage & Handling

• Storage: store at low temperature,keep away from direct sunlight | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Hedgehog, Hh Ag1.5, HhAg1.5, Hh-Ag1.5, Hh-Ag-1.5, SAG 1.5, SAG1.5, SAG-1.5, Smoothened

Compound Identifiers

PubChem CID

44195701