SMN2 Antibody

• Catalog Number: orb1260882
• Category: Antibodies
• Description: SMN2 Antibody
• Target: SMN2
• Clonality: Polyclonal
• Isotype: IgG
• Conjugation: Unconjugated
• Reactivity: Human, Mouse, Rat
• Form/Appearance: Liquid
• Concentration: batch dependent
• Buffer/Preservatives: Liquid
• Purification: Affinity purification
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-197 of human SMN2 (NP_059107.1).
• UniProt ID: Q16637
• MW: Observed: 35kDa
• Tested applications: IF, IHC, IP, WB
• Application notes: WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200IP: 1:20 - 1:50
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: SMN1, BCD541, SMA, SMA1, SMA2, SMA3, SMA4, SMA@, S
• Note: For research use only

Western blot analysis of extracts of various cell lines, using SMN2 antibody (orb1260882) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Immunohistochemistry of paraffin-embedded rat heart using SMN2 antibody (orb1260882) (40x lens).

Immunohistochemistry of paraffin-embedded human prostate using SMN2 antibody (orb1260882) (40x lens).

Immunofluorescence analysis of U2OS cells using SMN2 antibody (orb1260882) at dilution of 1:100. Blue: DAPI for nuclear staining.