SMER28

SKU: orb1304482

Description

SMER28 is a small-molecule autophagy enhancer that operates via an mTOR-independent pathway, distinct from rapamycin. It has been shown to reduce amyloid-beta peptide accumulation in cellular models, making it a useful research tool for studying neurodegenerative diseases and autophagy mechanisms in vitro.

Research Area

Cell Biology

Key Properties

• CAS Number: 307538-42-7
• MW: 264.12
• Purity: >99.99% (May vary between batches)
• Formula: C₁₁H₁₀BrN₃
• SMILES: BrC1=CC=C2N=CN=C(NCC=C)C2=C1
• Target: Autophagy
• Solubility: DMSO:60 mg/mL (227.17 mM);10% DMSO+40% PEG300+5% Tween 80+45% Saline:2 mg/mL (7.57 mM)

Bioactivity

• In Vitro:
  SMER28 is a positive regulator of autophagy in a mechanism independent from the mTOR pathway. It increases autophagosome synthesis and enhances the clearance of model autophagy substrates such as A53T a-synuclein and mutant huntingtin fragments. A marked decrease in Aβ/APP-CTF levels and enhancement for degradation of Aβ/APP-CTF via autophagy is also induced by SMER28. APP-CTF and LC3-II are cocompartmentalized on SMER28 treatment.
• Cell Research:
  MEF cells overexpressing βCTF are treated with autophagy-enhancing compounds (10 μM) for 16 h. Cell lysates are analyzed by SDS-PAGE and Western blotting using anti-βCTF (6E10) and anti-tubulin antibodies.(Only for Reference)

Storage & Handling

• Storage: Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Inhibitor, inhibit, Autophagy, SMER 28, SMER28, SMER-28

Compound Identifiers

PubChem CID

1560402