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SMARCC1 Antibody
SKU: orb1274438
Featured
Description
Images & Validation
−Item 1 of 4
| Tested Applications | IHC, IP, WB |
|---|---|
| Reactivity | Human |
| Application Notes |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | Recombinant fusion protein containing a sequence corresponding to amino acids 80-320 of human SMARCC1 (NP_003065.3). |
| Target | SMARCC1 |
| Molecular Weight | Observed: 155kDa |
| Purification | Affinity purification |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Liquid |
| Buffer/Preservatives | PBS with 0.02% sodium azide, 50% glycerol, pH 7.3. |
| Concentration | batch dependent |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−SMARCC1, Rsc8, SRG3, SWI3, BAF155, CRACC1
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Western blot analysis of extracts of various cell lines, using SMARCC1 antibody (orb1274438) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 120s.
Western blot analysis of extracts from normal (control) and SMARCC1 knockout (KO) HeLa cells, using SMARCC1 antibody (orb1274438) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 15s.
Immunohistochemistry of paraffin-embedded human kidney cancer using SMARCC1 antibody (orb1274438) at dilution of 1:200 (40x lens).
Immunoprecipitation analysis of 200 ug extracts of 293T cells using 1 ug SMARCC1 antibody (orb1274438). Western blot was performed from the immunoprecipitate using SMARCC1 antibody (orb1274438) at a dilution of 1:1000.
Quick Database Links
Gene Symbol
SMARCC1
UniProt
UniProt Details
− No UniProt data available
Documents Download
Datasheet
Product Information
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC
Immunohistochemistry
IP
Immunoprecipitation
SMARCC1 Antibody (orb1274438)
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