SMARCA4 Antibody

• Catalog Number: orb1240006
• Category: Antibodies
• Description: SMARCA4 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, IF, IHC-P, WB
• Predicted Reactivity: Bovine
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: SMARCA4 antibody was raised against a 19 amino acid peptide near the carboxy terminus of human SMARCA4.The immunogen is located within amino acids 1420 - 1470 of SMARCA4.
• Concentration: 1 mg/mL
• Dilution range: SMARCA4 antibody can be used for detection of SMARCA4 by Western blot at 1 - 2 μg/ml. Antibody can also be used for Immunohistochemistry at 2 μg/mL. For Immunoflorescence start at 20 μg/mL.Antibody validated: Western Blot in human samples; Immunohistochemistry in mouse and rat samples and Immunofluorescence in mouse samples. All other applications and species not yet tested.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: Predicted: 185 kDa Observed: 200 kDa
• Target: SMARCA4
• UniProt ID: P51532
• NCBI: NP_001122321
• Storage: SMARCA4 antibody can be stored at 4°C for three months and -20°C, stable for up to one year.
• Buffer/Preservatives: SMARCA4 Antibody is supplied in PBS containing 0.02% sodium azide.
• Alternative names: SMARCA4 Antibody: BRG1, SNF2, SWI2, MRD16, RTPS2,
• Note: For research use only
• Application notes: SMARCA4 antibody can be used for detection of SMARCA4 by Western blot at 1 - 2 μg/ml. Antibody can also be used for Immunohistochemistry at 2 μg/mL. For Immunoflorescence start at 20 μg/mL.Antibody validated: Western Blot in human samples; Immunohistochemistry in mouse and rat samples and Immunofluorescence in mouse samples. All other applications and species not yet tested.
• Expiration Date: 12 months from date of receipt.

Western blot analysis of SMARCA4 in SK-N-SH cell lysate with SMARCA4 antibody at 1 µg/ml.

Immunohistochemistry of SMARCA4 in rat brain tissue with SMARCA4 antibody at 5 µg/mL.

Immunohistochemistry of SMARCA4 in rat brain tissue with SMARCA4 antibody at 5 µg/mL.

Immunohistochemistry of SMARCA4 in mouse testis tissue with SMARCA4 antibody at 2 µg/ml.

Immunofluorescence of SMARCA4 in mouse testis tissue with SMARCA4 antibody at 20 µg/ml. Green: SMARCA4 Antibody (orb1240006) Blue: DAPI staining.