Smad4 Rabbit mAb [KD Validated]

• Catalog Number: orb1951975
• Category: Antibodies
• Description: Smad4 transcription factor that mediates signal transduction by the transforming growth factor superfamily. The common smad (co-smad). Binds directly to consensus DNA-binding elements in the promoters of target genes. Promotes binding of the Smad2/Smad4/Fast-1 complex to DNA and provides an activation function required for Smad1 or Smad2 to stimulate transcription.
• Species/Host: Rabbit
• Clonality: Recombinant
• Tested applications: IHC, WB
• Reactivity: Human, Mouse, Rat
• Isotype: Rabbit IgG
• Immunogen: A synthesized peptide derived from human Smad4
• Antibody Type: Recombinant Antibody
• Dilution range: WB 1:500~1:2000 IHC 1:50~1:200
• Form/Appearance: Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Conjugation: Unconjugated
• UniProt ID: Q13485
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: MAD homolog 4; MAD, mothers against decapentaplegi
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.

Immunohistochemical analysis of paraffin-embedded human breast cancer, using Smad4 Antibody.

Western blot analysis of SMAD4 expression in (1) SH-SY5Y cell lysate; (2) NIH/3T3 cell lysate.