SMAD3-S208 Antibody

SKU: orb1927597

Description

SMAD3-S208 Antibody

Research Area

Cancer Biology

Images & Validation

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Item 1 of 4

Tested Applications	FC, IF, IHC-P, WB
Dilution Range	WB - 1:1000, IHC-P - 1:100-500, IF - 1:10-50, FC - 1:10-50
Reactivity	Human, Mouse, Rat
Predicted Reactivity	Gallus, Porcine

Key Properties

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Host	Rabbit
Clonality	Polyclonal
Isotype	Rabbit IgG
Molecular Weight	48081 Da
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
Form/Appearance	Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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MADH3

Quality Guarantee

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SMAD3 Antibody (S208) western blot analysis in HepG2 and mouse C2C12 cell line lysates (35 ug/lane).This demonstrates the SMAD3 antibody detected the SMAD3 protein (arrow).

Confocal immunofluorescent analysis of SMAD3-S208 Antibody with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).

SMAD3-S208 Antibody flow cytometric analysis of MDA-MB231 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

SMAD3-S208 Antibody IHC analysis in formalin fixed and paraffin embedded brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the SMAD3-S208 Antibody for immunohistochemistry. Clinical relevance has not been evaluated.