Smad3 Rabbit mAb

• Catalog Number: orb1565428
• Category: Antibodies
• Description: Smad3 Rabbit mAb
• Clonality: Monoclonal
• Clone Number: R09-7H4
• Tested applications: ICC, IF, IHC-Fr, IHC-P, WB
• Reactivity: Human, Rat
• Isotype: IgG
• Immunogen: A synthetic peptide of human Smad3
• Antibody Type: Primary Antibody
• Concentration: 0.3 mg/ml
• Dilution range: WB: 1/500-1/1000 IHC: 1/50-1/100 IF: 1/50-1/200
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: Calculated MW: 48 kDa; Observed MW: 52 kDa
• Target: SMAD3
• UniProt ID: P84022
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40%Glycerol, 0.01% sodium azide and 0.05% rAlbumin.
• Alternative names: SMAD3; MADH3; Mothers against decapentaplegic homo
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Immunocytochemistry analysis of Smad3 (green) in A549 using Smad3 antibody, and DAPI (blue).

Western blot analysis of Smad3 in Jurkat, C6, Hela lysates using Smad3 antibody.

Immunohistochemistry analysis of paraffin-embedded Human breast cancer tissue using Smad3 antibody. High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.