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SMAD3 (phospho-T179) antibody

Catalog Number: orb345687

DispatchUsually dispatched within 5-10 working days
$ 292.00
Catalog Numberorb345687
CategoryAntibodies
DescriptionSMAD3 (phospho-T179) antibody
Species/HostRabbit
ClonalityPolyclonal
Tested applicationsELISA, WB
ReactivityMouse
IsotypeIgG
ImmunogenAnti-SMAD3 pT179 antibody was prepared by repeated immunizations with a synthetic peptide corresponding to an internal region of human Smad3 protein surrounding amino acid residue 179.
Concentration1.1 mg/mL
Dilution rangeELISA: 1:15,000-1:75,000, WB: 1:1,000
Form/AppearanceLiquid (sterile filtered)
PurityAnti-SMAD3 pT179 affinity-purified antibody is directed against the phosphorylated form of human Smad3 protein at the pT179 residue. The product was affinity purified from monospecific antiserum by immunoaffinity purification. Antiserum was first purified against the phosphorylated form of the immunizing peptide. The resultant affinity purified antibody was then cross adsorbed against the non-phosphorylated form of the immunizing peptide. Reactivity occurs against human Smad3 pT179 protein and the antibody is specific for the phosphorylated form of the protein. Reactivity with non-phosphorylated human Smad3 is minimal by ELISA and western blot. Expect reactivity against phosphorylated Smad2. Reactivity against other phosphorylated Smad family members is not known. A BLAST analysis was used to suggest cross reactivity with Smad3 from human, mouse, rat, pig, dog, and marmoset based on 100% sequence homology with the immunogen. Reactivity against homologues from other sources is not known.
ConjugationUnconjugated
UniProt IDP84022
NCBINP_005893
StorageStore vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
Buffer/Preservatives0.01% (w/v) Sodium Azide
Alternative namesrabbit anti-SMAD3 pT179 antibody, SMAD-3, SMAD 3,
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NoteFor research use only
Application notesAnti-SMAD3 pT179 has been tested for use in ELISA and by western blot, and suitable by immunohistochemistry. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 48.1 kDa in size corresponding to human phosphorylated Smad3 protein by western blotting in the appropriate stimulated tissue or cell lysate or extract.
Expiration Date12 months from date of receipt.
SMAD3 (phospho-T179) antibody

Western blot analysis of mouse mammary epithelial cells using SMAD3 (phospho-T179) antibody

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