Smad3 (Phospho-Ser213) Antibody

• Catalog Number: orb771752
• Category: Antibodies
• Description: Rabbit polyclonal antibody to Smad3 (Phospho-Ser213)
• Clonality: Polyclonal
• Tested applications: ELISA, WB
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: Synthesized phospho derived from human Smad3 (Phospho-Ser213)
• Concentration: 1 mg/ml
• Dilution range: WB 1:500-2000;IHC-p 1:50-300; ELISA 2000-20000
• Conjugation: Unconjugated
• MW: 50
• Source: Rabbit
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS with 0.02% sodium azide and 50% glycerol pH 7.4.
• Alternative names: Anti-Mothers against decapentaplegic homolog 3 (MA
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western blot analysis of various lysate, antibody was diluted at 1000. Secondary antibody was diluted at 1:20000.

Immunohistochemical analysis of paraffin-embedded human Colon cancer. 1, Antibody was diluted at 1:200 (4°C overnight). 2, Tris-EDTA, pH9.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 45min).