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Catalog Number | orb764368 |
---|---|
Category | Antibodies |
Description | Rabbit polyclonal antibody to Smad3 (phospho-Ser208) |
Clonality | Polyclonal |
Isotype | IgG |
Conjugation | Unconjugated |
Reactivity | Human, Mouse, Rat |
Concentration | 1 mg/ml |
Buffer/Preservatives | PBS with 0.02% sodium azide and 50% glycerol pH 7.4. |
Immunogen | The antiserum was produced against synthesized peptide derived from human Smad3 around the phosphorylation site of Ser208. AA range:174-223 |
MW | 40 |
Tested applications | ELISA, IHC-P, WB |
Dilution range | Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/10000. Not yet tested in other applications. |
Source | Rabbit |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Alternative names | Anti-SMAD3 antibody, anti-MADH3 antibody, anti-Mot Read more... |
Note | For research use only |
Western Blot analysis of various cells using Phospho-Smad3 (S208) Polyclonal Antibody.
Western Blot analysis of Jurkat cells using Phospho-Smad3 (S208) Polyclonal Antibody.
Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using Smad3 (Phospho-Ser208) Antibody.
Immunohistochemistry analysis of paraffin-embedded human colon carcinoma, using Smad3 (Phospho-Ser208) Antibody. The picture on the right is blocked with the phospho peptide.
ELISA, FC, ICC, IF, IHC-Fr, IHC-P, WB | |
Human, Rat | |
Polyclonal | |
Unconjugated |
FC, IF | |
Mouse | |
Human, Rat | |
Rabbit | |
Polyclonal | |
Pacific Blue |
FC, IF | |
Mouse | |
Human, Rat | |
Rabbit | |
Polyclonal | |
BF700 |
WB | |
Mouse | |
Human, Rat | |
Rabbit | |
Polyclonal | |
IRDye800 |