Smad3 (phospho Ser204) rabbit pAb

• Catalog Number: orb764310
• Category: Antibodies
• Description: Rabbit polyclonal antibody to Smad3 (phospho-Ser204)
• Clonality: Polyclonal
• Isotype: IgG
• Conjugation: Unconjugated
• Reactivity: Human, Mouse, Rat
• Concentration: 1 mg/ml
• Buffer/Preservatives: PBS with 0.02% sodium azide and 50% glycerol pH 7.4.
• Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• Immunogen: The antiserum was produced against synthesized peptide derived from human Smad3 around the phosphorylation site of Ser204. AA range:170-219
• MW: 48
• Tested applications: ELISA, IF, IHC-P, WB
• Dilution range: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/20000. Not yet tested in other applications.
• Source: Rabbit
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: Anti-SMAD3 antibody, anti-MADH3 antibody, anti-Mot
• Note: For research use only

Immunofluorescence analysis of Hela cell. 1, Smad3 (phospho Ser204) Polyclonal Antibody (red) was diluted at 1:200 (4°C overnight). LC3A mouse Monoclonal Antibody (5G10) (green) was diluted at 1:200 (4°C overnight). 2, Goat Anti Rabbit Alexa Fluor 594 was diluted at 1:1000 (room temperature, 50min). Goat Anti Mouse Alexa Fluor 488 was diluted at 1:1000 (room temperature, 50min).

Western Blot analysis of various cells using Phospho-Smad3 (S204) Polyclonal Antibody diluted at 1:500.

Western Blot analysis of MCF-7 cells using Phospho-Smad3 (S204) Polyclonal Antibody diluted at 1:500.

Immunohistochemical analysis of paraffin-embedded Human brain. Antibody was diluted at 1:100 (4°C overnight). High-pressure and temperature Tris-EDTA, pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.