SMAD3 Antibody (S213)

SKU: orb2649341

Description

Research Area

Cancer Biology

Images & Validation

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Item 1 of 5

Tested Applications	FC, IF, IHC-P, WB
Dilution Range	WB - 1:1000, IHC-P - 1:100-500, IF - 1:10-50, FC - 1:10-50
Reactivity	Human
Predicted Reactivity	Gallus, Mouse, Porcine, Rat

Key Properties

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Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Polyclonal
Isotype	Rabbit IgG
Molecular Weight	48081 Da
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/Appearance	Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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MADH3

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Applications:	FC, IF, IHC-Fr, IHC-P
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Sizes Available: 50 μl, 100 μl, 200 μl

Item 1 of 4
SMAD3 (Phospho-S213) Rabbit Polyclonal Antibody [orb315707]
Applications:
IF, IHC, WB
Reactivity:
Gallus, Human, Mouse, Porcine, Rat
Species/Host:
Rabbit
Clonality:
Polyclonal
Conjugation:
Unconjugated
Sizes Available: 100 μl, 200 μl, 30 μl, 50 μl
Item 1 of 3
Phospho-SMAD3(S213) Antibody [orb1931224]
Applications:
WB
Predicted Reactivity:
Gallus, Mouse, Porcine, Rat
Reactivity:
Human
Species/Host:
Rabbit
Clonality:
Polyclonal
Conjugation:
Unconjugated
Sizes Available: 100 μl, 50 μl
SMAD3 Antibody [orb673888]
Applications:
ELISA, IHC, WB
Reactivity:
Human, Mouse, Rat
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Rabbit
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Polyclonal
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Unconjugated
Sizes Available: 100 μg, 50 μg
Smad3 (phospho-S213) polyclonal antibody [orb642407]
Applications:
IF, WB
Reactivity:
Human, Mouse, Rat
Species/Host:
Rabbit
Clonality:
Polyclonal
Conjugation:
Unconjugated
Sizes Available: 25 μl, 200 μl, 100 μl, 50 μl

Quality Guarantee

Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our Terms & Conditions and Buying Guide. Contact us at [email protected].

Confocal immunofluorescent analysis of SMAD3 Antibody (S213) with A549 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).

Confocal immunofluorescent analysis of SMAD3 Antibody (S213) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).

SMAD3 Antibody (S213) flow cytometric analysis of A549 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

SMAD3 Antibody (S213) immunohistochemistry analysis in formalin fixed and paraffin embedded human thyroid tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of SMAD3 Antibody (S213) for immunohistochemistry. Clinical relevance has not been evaluated.

SMAD3 Antibody (S213) western blot analysis in A549 cell line lysates (35 ug/lane). This demonstrates the SMAD3 antibody detected the SMAD3 protein (arrow).

Quick Database Links

UniProt

P84022

UniProt Details

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No UniProt data available

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Protocol Information

Western Blot (IB, immunoblot)

View Protocol

IHC-P

Immunohistochemistry Paraffin

Flow Cytometry

Immunofluorescence