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SMAD3 Antibody
SKU: orb69286
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Description
Images & Validation
−Item 1 of 2
| Tested Applications | ICC, WB |
|---|---|
| Dilution Range | WB: 1/500 - 1/2000; IF/ICC: 1/200 - 1/1000; ELISA: 1/10000 |
| Reactivity | Human, Mouse |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | Mouse IgG1 |
| Clone No. | 5G11E8 |
| Immunogen | Purified recombinant fragment of human SMAD3 expressed in E. Coli. |
| Target | Disease |
| Molecular Weight | 48kDa |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Buffer/Preservatives | Purified antibody in PBS with 0.05% sodium azide. |
| Concentration | Approximately 1mg/ml (varies from batch to batch). Please inquire for precise concentration. |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−MADH3; JV15-2; HSPC193; HsT17436; MGC60396; DKFZp586N0721; DKFZp686J10186; SMAD3
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Western blot analysis of A549 (lane1), Hela (lane2), Jurkat (lane3), PC-2 (lane4) and NIH/3T3 (lane5) cell lysate using SMAD3 antibody.
Immunofluorescence analysis of NIH/3T3 cells using SMAD3 antibody
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Protocol Information
WB
Western Blot (IB, immunoblot)
ICC
Immunocytochemistry
SMAD3 Antibody (orb69286)
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