SMAD3 (Ab-425) Antibody

• Catalog Number: orb684747
• Category: Antibodies
• Description: This SMAD3 (Ab-425) Antibody is an unconjugated polyclonal product. It tagets SMAD3 using a Peptide sequence around aa.423~427 (C-S-S-V-S) derived from Human Smad3 as the immunogen. This antibody is suitable for ELISA, IF, IHC.
• Target: SMAD3
• Clonality: Polyclonal
• Species/Host: Rabbit
• Isotype: IgG
• Conjugation: Unconjugated
• Reactivity: Human, Mouse, Rat
• Form/Appearance: liquid
• Buffer/Preservatives: Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Purification: Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.
• Immunogen: Peptide sequence around aa.423~427 (C-S-S-V-S) derived from Human Smad3.
• UniProt ID: P84022
• Tested applications: ELISA, IF, IHC
• Dilution range: IHC-P: 1: 50-1: 100, IF: 1: 100-1: 200
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: Anti-JV15-2 antibody, anti-MAD-3 antibody, anti-MA
• Research Area: Signal Transduction
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Immunofluorescence analysis of Hela cells using SMAD3 (Ab-425) antibody

Immunohistochemical staining of human breast carcinoma tissue using SMAD3 (Ab-425) antibody