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Description
Research Area
Images & Validation
−| Tested Applications | ELISA, IHC, WB |
|---|---|
| Dilution Range | WB: 1: 500-1: 3000, IHC-P: 1: 50-1: 100 |
| Reactivity | Human, Mouse, Rat |
Key Properties
−| Host | Rabbit |
|---|---|
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | Synthesized non-phosphopeptide derived from Human Smad3 around the phosphorylation site of serine 204 (A-G-S(p)-P-N). |
| Target | SMAD3 |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | liquid |
| Buffer/Preservatives | Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
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Immunohistochemical staining of human colon Carcinoma Tissue using SMAD3 (Ab-204) antibody

Western blot analysis of HUVEC cells using SMAD3 (Ab-204) antibody
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SMAD3 Antibody (orb684801)
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