SMAD2/SMAD3 Antibody

SKU: orb1255339

Description

SMAD2/SMAD3 Antibody

Research Area

Cancer Biology, Cell Biology, Epigenetics & Chromatin, Signal Transduction

Images & Validation

• Tested Applications: IF, IHC, WB
• Reactivity: Human, Mouse, Rat
• Application Notes:
  WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-230 of human SMAD3 (NP_005893.1).
• Target: SMAD2/SMAD3
• Molecular Weight: Observed: 56-60kDa
• Purification: Affinity purification
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: PBS with 0.02% sodium azide, 50% glycerol, pH 7.3.
• Concentration: batch dependent
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Western blot analysis of extracts of HeLa cells, using SMAD2/SMAD3 antibody (orb1255339) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.

Immunohistochemistry of paraffin-embedded human kidney using SMAD2/SMAD3 antibody (orb1255339) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded human gastric using SMAD2/SMAD3 antibody (orb1255339) at dilution of 1:100 (40x lens).

Immunofluorescence analysis of U2OS cells using SMAD2/SMAD3 antibody (orb1255339) at dilution of 1:100. Blue: DAPI for nuclear staining.

Quick Database Links

Gene Symbol

SMAD2/SMAD3

UniProt

P84022, Q15796