Smad2 (Phospho-Ser250) Rabbit mAb [KD Validated]

• Catalog Number: orb1727742
• Category: Antibodies
• Description: The protein encoded by this gene belongs to the SMAD, a family of proteins similar to the gene products of the Drosophila gene 'mothers against decapentaplegic' (Mad) and the C. elegans gene Sma. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways.
• Species/Host: Rabbit
• Clonality: Recombinant
• Tested applications: FC, WB
• Reactivity: Human, Mouse, Rat
• Isotype: Rabbit IgG
• Immunogen: A synthesized peptide derived from human Smad2
• Antibody Type: Recombinant Antibody
• Dilution range: WB:1:1000~1:2000FC:1:20
• Form/Appearance: Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Conjugation: Unconjugated
• MW: 58kDa
• UniProt ID: Q15796
• Source: Recombinant Rabbit
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: JV18-1, MADH2, MADR2, Mad-related protein 2, Mothe
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.

Western blot analysis of Phospho-Smad2 (S250) expression in (1) HeLa cell treated with alkaline phosphatase lysate; (2) HeLa cell lysate.