SMAD2 Antibody

• Catalog Number: orb1263277
• Category: Antibodies
• Description: SMAD2 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, WB
• Predicted Reactivity: Bovine, Mouse, Rat, Zebrafish
• Reactivity: Human
• Isotype: Rabbit Ig
• Immunogen: This SMAD2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 201-230 amino acids from human SMAD2.
• Concentration: batch dependent
• Dilution range: For IF starting dilution is: 1:200For WB starting dilution is: 1:1000
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 52 kDa
• Target: SMAD2
• UniProt ID: Q15796
• NCBI: Q15796
• Storage: Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
• Buffer/Preservatives: Supplied in PBS with 0.09% (W/V) sodium azide.
• Alternative names: Mothers against decapentaplegic homolog 2, MAD hom
• Note: For research use only
• Application notes: For IF starting dilution is: 1:200For WB starting dilution is: 1:1000
• Expiration Date: 12 months from date of receipt.

Fluorescent confocal image of SY5Y cells stained with SMAD2 (T220) antibody. SY5Y cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with SMAD2 (T220) primary antibody (1:200, 2 h at room temperature). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 ug/ml, 5 min). Note the highly specific localization of the SMAD2 mainly to the nucleus.

Western blot analysis of SMAD2 Antibody (T220) in NCI-H460 cell line lysates (35 ug/lane)