SMAD2 Antibody, KO Validated

• Catalog Number: orb1274423
• Category: Antibodies
• Description: SMAD2 Antibody, KO Validated
• Target: SMAD2
• Clonality: Polyclonal
• Isotype: IgG
• Conjugation: Unconjugated
• Reactivity: Human, Mouse, Rat
• Form/Appearance: Liquid
• Concentration: batch dependent
• Buffer/Preservatives: Liquid
• Purification: Affinity purification
• Immunogen: A synthetic peptide corresponding to a sequence within amino acids 1-100 of human SMAD2 (NP_001003652.1).
• UniProt ID: Q15796
• MW: Observed: 60kDa
• Tested applications: IF, IHC, WB
• Application notes: WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: SMAD2, JV18, MADH2, MADR2, JV18-1, hMAD-2, hSMAD2
• Note: For research use only

Western blot analysis of extracts of various cell lines, using SMAD2 antibody (orb1274423) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Western blot analysis of extracts from normal (control) and SMAD2 knockout (KO) HeLa cells, using SMAD2 antibody (orb1274423) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.

Immunohistochemistry of paraffin-embedded mouse spleen using SMAD2 antibody (orb1274423) at dilution of 1:100 (40x lens).

Immunofluorescence analysis of HeLa cells using SMAD2 antibody (orb1274423) at dilution of 1:100. Blue: DAPI for nuclear staining.

Immunofluorescence analysis of NIH/3T3 cells using SMAD2 antibody (orb1274423) at dilution of 1:100. Blue: DAPI for nuclear staining.

Immunofluorescence analysis of PC-12 cells using SMAD2 antibody (orb1274423) at dilution of 1:100. Blue: DAPI for nuclear staining.