SMAD1 Antibody

• Catalog Number: orb1261320
• Category: Antibodies
• Description: SMAD1 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, IHC, WB
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 20-240 of human SMAD1 (NP_001003688.1).
• Concentration: batch dependent
• Dilution range: WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: Observed: 54kDa
• Target: SMAD1
• UniProt ID: Q15797
• Storage: Store at -20°C. Avoid freeze / thaw cycles.
• Buffer/Preservatives: PBS with 0.02% sodium azide, 50% glycerol, pH 7.3.
• Alternative names: SMAD1, BSP1, JV4-1, JV41, MADH1, MADR1, BSP-1
• Note: For research use only
• Application notes: WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200
• Expiration Date: 12 months from date of receipt.

Western blot analysis of extracts of various cell lines, using SMAD1 antibody at 1:1000 dilution.

Immunohistochemistry of paraffin-embedded rat brain using SMAD1 antibody at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded human kidney using SMAD1 antibody at dilution of 1:100 (40x lens).

Immunofluorescence analysis of HeLa cells using SMAD1 antibody. Blue: DAPI for nuclear staining.