SMAC Antibody (C-term)

SKU: orb1929782

Description

Purified Rabbit Polyclonal Antibody (Pab)

Research Area

Cell Biology

Images & Validation

• Tested Applications: FC, IHC-P, WB
• Dilution Range: WB - 1:1000, IHC-P - 1:50-100, FC - 1:10-50
• Reactivity: Human

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Clone No.: BTD42977
• Immunogen: This SMAC antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 211-239 amino acids from the C-terminal region of human SMAC. Antigen Region: 211-239 aa.
• Target: DIABLO (HGNC:21528)
• Molecular Weight: 27131 Da
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Diablo homolog, mitochondrial, Direct IAP-binding protein with low pI, Second mitochondria-derived activator of caspase, Smac, DIABLO, SMAC

SMAC Antibody (C-term) flow cytometric analysis of MDA-MB231 cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Formalin-fixed and paraffin-embedded human testis tissue reacted with SMAC Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Western blot analysis of SMAC Antibody (C-term) in T47D cell line lysates (35 ug/lane). SMAC (arrow) was detected using the purified Pab.

All lanes: Anti-SMAC Antibody (C-term) at 1:1000 dilution. Lane 1: Hela whole cell lysate. Lane 2: Jurkat whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 27 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Quick Database Links

Gene Symbol

DIABLO (HGNC:21528)

UniProt

Q9NR28

RefSeq (Protein):NP_001265232.1, NP_001265271.1, NP_001265233.1, NP_001265231.1, NP_620308.1, NP_063940.1