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Catalog Number | orb1931752 |
---|---|
Category | Antibodies |
Description | Purified Rabbit Polyclonal Antibody (Pab) |
Species/Host | Rabbit |
Clonality | Polyclonal |
Clone Number | RB01398 |
Tested applications | IF, IHC-P, WB |
Reactivity | Human, Mouse, Rat |
Isotype | Rabbit IgG |
Antibody Type | Primary Antibody |
Dilution range | IF: 1:10~50, WB: 1:2000, WB: 1:1000, IHC-P: 1:10~50 |
Form/Appearance | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
Conjugation | Unconjugated |
MW | 29986 Da |
Target | This SLUG antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 98-129 amino acids from the Central region of human SLUG. |
UniProt ID | O43623 |
NCBI | NP_003059.1 |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles |
Alternative names | Zinc finger protein SNAI2, Neural crest transcript Read more... |
Note | For research use only |
Expiration Date | 12 months from date of receipt. |
Confocal immunofluorescent analysis of SLUG Antibody (Center) with HepG2 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red). DAPI was used to stain the cell nuclear (blue).
Formalin-fixed and paraffin-embedded human Breast tissue reacted with SLUG Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
SLUG Antibody (Center) is used in Western blot to detect SLUG in mouse heart tissue lysate.
All lanes: Anti-SLUG Antibody (Center) at 1:2000 dilution. Lane 1: mouse heart lysate. Lane 2: PC-12 whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 30 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.
IF, IHC-P, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |