SLUG Antibody (Center)

• Catalog Number: orb1931752
• Category: Antibodies
• Description: Purified Rabbit Polyclonal Antibody (Pab)
• Target: This SLUG antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 98-129 amino acids from the Central region of human SLUG.
• Clonality: Polyclonal
• Species/Host: Rabbit
• Isotype: Rabbit IgG
• Conjugation: Unconjugated
• Reactivity: Human, Mouse, Rat
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• UniProt ID: O43623
• MW: 29986 Da
• Tested applications: IF, IHC-P, WB
• Dilution range: IF: 1:10~50, WB: 1:2000, WB: 1:1000, IHC-P: 1:10~50
• Antibody Type: Primary Antibody
• Clone Number: RB01398
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: Zinc finger protein SNAI2, Neural crest transcript
• Note: For research use only
• NCBI: NP_003059.1

Confocal immunofluorescent analysis of SLUG Antibody (Center) with HepG2 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red). DAPI was used to stain the cell nuclear (blue).

Formalin-fixed and paraffin-embedded human Breast tissue reacted with SLUG Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

SLUG Antibody (Center) is used in Western blot to detect SLUG in mouse heart tissue lysate.

All lanes: Anti-SLUG Antibody (Center) at 1:2000 dilution. Lane 1: mouse heart lysate. Lane 2: PC-12 whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 30 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.