SLCO1B3 Antibody

SKU: orb1249434

Description

SLCO1B3 Antibody

Images & Validation

• Tested Applications: ELISA, FC, IF, WB
• Reactivity: Human
• Predicted Reactivity: Canine, Mouse
• Application Notes:
  Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 75kDa band observed in lysates of cell line HEK293 and A431 (calculated MW of 74.2kDa according to NP_001336849.1 and 77.4kDa acording to NP_062818.1). Recommended concentration: 0.5-1ug/ml. Primary incubation 1 hour at room temperature.Immunofluorescence: Strong expression of the protein seen in the membranes of A431 cells. Recommended concentration: 10ug/ml. Flow Cytometry: Flow cytometric analysis of HepG2 cells. Recommended concentration: 10ug/ml.

Key Properties

• Antibody Type: Primary Antibody
• Host: Goat
• Clonality: Polyclonal
• Immunogen: The immunogen for this antibody is: C-NGTSPEIVEKD
• Target: SLCO1B3
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Concentration: 500 ug/mL
• Disclaimer: For research use only

Alternative Names

SLCO1B3, solute carrier organic anion transporter family, member 1B3, LST-3TM13, LST3, OATP1B3, OATP8, SLC21A8, liver-specific organic anion transporter 3TM13, organic anion transporter LST-3c, solute carrier family 21 (organic anion transporter), member 8

orb1249434 (1 ug/ml) staining of HEK293 (A) and A431 (B) cell lysate (35 ug protein in RIPA buffer). Detected by chemiluminescence.

orb1249434 Immunofluorescence analysis of paraformaldehyde fixed A431 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (2 ug/ml), showing membrane staining. The nuclear stain is DAPI.

orb1249434 Flow cytometric analysis of paraformaldehyde fixed HepG2 cells (blue line), permeabilized with 0.5% Triton. Primary incubation overnight (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (1 ug/ml). IgG control: Unimmunized goat IgG (black line).

Quick Database Links

Gene Symbol

SLCO1B3

UniProt

Q9NPD5

RefSeq (Protein):NP_001336849.1, NP_062818.1