SLC6A14 Antibody (C-term)

SKU: orb1937035

Description

Affinity Purified Rabbit Polyclonal Antibody (Pab)

Research Area

Signal Transduction

Images & Validation

• Tested Applications: IHC-P, WB
• Dilution Range: WB - 1:1000, IHC-P-Leica - 1:500
• Reactivity: Human

Key Properties

• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Clone No.: RB32462
• Immunogen: This SLC6A14 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 602-631 amino acids from the C-terminal region of human SLC6A14. Antigen Region: 602-631 aa.
• Target: SLC6A14 (HGNC:11047)
• Molecular Weight: 72153 Da
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Sodium- and chloride-dependent neutral and basic amino acid transporter B(0+), Amino acid transporter ATB0+, Solute carrier family 6 member 14, SLC6A14

All lanes: Anti-SLC6A14 Antibody (C-term) at 1:1000 dilution. Lane 1: Human lung lysate. Lane 2: Hela whole cell lysate. Lane 3: HepG2 whole cell lysate. Lane 4: HACAT whole cell lysate. Lysates/proteins at 20 ug per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 80 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

All lanes: Anti-SLC6A14 Antibody (C-term) at 1:1000 dilution. Lane 1: Human lung lysate. Lane 2: Human breast lysate.Lysates/proteins at 20 ug per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 80 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Immunohistochemical analysis of paraffin-embedded human lung tissue performed on the Leica BOND RXm. Tissue was fixed with formaldehyde at room temperature, antigen retrieval was by heat mediation with a EDTA buffer (pH9.0). Samples were incubated with primary antibody (1:500) for 1 hours at room temperature. A undiluted biotinylated CRF Anti-Polyvalent HRP Polymer antibody was used as the secondary Antibody.

SLC6A14 Antibody (C-term) western blot analysis in HL-60 cell line lysates (35 ug/lane). This demonstrates the SLC6A14 antibody detected the SLC6A14 protein (arrow).

Quick Database Links

Gene Symbol

SLC6A14 (HGNC:11047)

UniProt

Q9UN76

RefSeq (Protein):NP_009162.1