SLC47A1 Antibody (C-term)

SKU: orb1930543

Description

Affinity Purified Rabbit Polyclonal Antibody (Pab)

Research Area

Metabolism Research, Signal Transduction

Images & Validation

• Tested Applications: IHC-P, WB
• Dilution Range: WB - 1:2000, IHC-P-Leica - 1:500
• Reactivity: Human

Key Properties

• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Clone No.: BTD48120
• Immunogen: This SLC47A1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 492-519 amino acids from the C-terminal region of human SLC47A1. Antigen Region: 492-519 aa.
• Target: SLC47A1 (HGNC:25588)
• Molecular Weight: 61922 Da
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Multidrug and toxin extrusion protein 1, MATE-1, hMATE-1, Solute carrier family 47 member 1, SLC47A1, MATE1

Immunohistochemical analysis of paraffin-embedded human kidney tissue was performed on the Leica BOND RXm. Tissue was fixed with formaldehyde at room temperature, antigen retrieval was by heat mediation with a EDTA buffer (pH9.0). Samples were incubated with primary antibody (1:500) for 1 hours at room temperature. A undiluted biotinylated CRF Anti-Polyvalent HRP Polymer antibody was used as the secondary antibody.

Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue was performed on the Leica BOND RXm. Tissue was fixed with formaldehyde at room temperature, antigen retrieval was by heat mediation with a EDTA buffer (pH9.0). Samples were incubated with primary antibody (1:500) for 1 hours at room temperature. A undiluted biotinylated CRF Anti-Polyvalent HRP Polymer antibody was used as the secondary antibody.

All lanes: Anti-SLC47A1 Antibody (C-term) at 1:2000 dilution. Lane 1: HepG2 whole cell lysate. Lane 2: A549 whole cell lysate. Lane 3: MDA-MB-453 whole cell lysate. Lane 4: 293 whole cell lysate. Lane 5: 293T/17 whole cell lysate. Lane 6: Hela whole cell lysate. Lane 7: T47D whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 62 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Quick Database Links

Gene Symbol

SLC47A1 (HGNC:25588)

UniProt

Q96FL8

RefSeq (Protein):NP_060712.2