SLC3A2 Antibody (C-term)

• Catalog Number: orb1939136
• Category: Antibodies
• Description: Affinity Purified Rabbit Polyclonal Antibody (Pab)
• Species/Host: Rabbit
• Clonality: Polyclonal
• Clone Number: RB22529
• Tested applications: IHC-P, WB
• Reactivity: Human
• Isotype: Rabbit IgG
• Antibody Type: Primary Antibody
• Dilution range: WB: 1:4000, WB: 1:4000, IHC-P: 1:25, IHC-P: 1:25
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Conjugation: Unconjugated
• MW: 67994 Da
• Target: This SLC3A2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 588-615 amino acids from the C-terminal region of human SLC3A2.
• UniProt ID: P08195
• NCBI: NP_001012682.1, NP_001013269.1, NP_002385.3, NP_001012680.1
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: 4F2 cell-surface antigen heavy chain, 4F2hc, 4F2 h
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

All lanes: Anti-SLC3A2 Antibody (C-term) at 1:4000 dilution. Lane 1: HL-60 whole cell lysate. Lane 2: HepG2 whole cell lysate. Lane 3: A431 whole cell lysate. Lane 4: Human kidney lysate. Lane 5: Human placenta lysate. Lane 6: Hela whole cell lysate. Lysates/proteins at 10 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 68 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

All lanes: Anti-SLC3A2 Antibody (C-term) at 1:4000 dilution. Lane 1: HL-60 whole cell lysate. Lane 2: HepG2 whole cell lysate. Lane 3: A431 whole cell lysate. Lane 4: Human kidney lysate. Lane 5: Human placenta lysate. Lane 6: Hela whole cell lysate.Lysates/proteins at 10 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 68 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Staining SLC3A2 in human kidney tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0.5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary Antibody.

Staining SLC3A2 in human placenta tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0.5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary Antibody.