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Description
Research Area
Epigenetics
Images & Validation
−Item 1 of 2
| Tested Applications | ELISA, WB |
|---|---|
| Dilution Range | WB: 1:500-1:2000 |
| Reactivity | Human, Mouse, Rat |
Key Properties
−| Host | Rabbit |
|---|---|
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | Recombinant fusion protein containing a sequence corresponding to amino acids 562-639 of human SLC34A1 (NP_003043.3). |
| Molecular Weight | 75-80kDa |
| Purity | Affinity purification |
| Purification | Affinity purification |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Buffer/Preservatives | PBS with 0.02% sodium azide,50% glycerol,pH7.3. |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−NPT2; FRTS2; SLC11; HCINF2; NAPI-3; NPTIIa; NPHLOP1; SLC17A2; SLC34A1
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Western blot analysis of Rat kidney, using SLC34A1 Rabbit pAb (orb541679) at 1:2000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.
Western blot analysis of various lysates, using SLC34A1 Rabbit pAb (orb541679) at 1:2000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.
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Protocol Information
WB
Western Blot (IB, immunoblot)
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
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Junying Zhang 1, Tiantian Che 2, Liting Wang 3, Wei Sun 3, Jing Zhao 3, Jiajia Chen 3, Yang Liu 3, Qi Pu 3, Yu Zhang 3, Jiani Li 4, Zhangfu Li 5, Zhaojing Zhu 6, Qihuan Fu 7, Xiaoyang Wang 8, Jiangbei Yuan Proteomics coupled transcriptomics reveals Slc34a1 and Slc34a3 downregulation as potential features of nephrotoxin-induced acute kidney injury J Proteomics, (2024)
Applications
WB
Reactivity
Mouse
SLC34A1 Rabbit pAb (orb541679)
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