SLC23A1 Antibody (N-term)

• Catalog Number: orb1937247
• Category: Antibodies
• Description: Affinity Purified Rabbit Polyclonal Antibody (Pab)
• Species/Host: Rabbit
• Clonality: Polyclonal
• Clone Number: RB32316
• Tested applications: IHC-P, WB
• Reactivity: Human
• Isotype: Rabbit IgG
• Antibody Type: Primary Antibody
• Dilution range: WB: 1:1000, WB: 1:1000-1:2000, IHC-P-Leica: 1:500
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Conjugation: Unconjugated
• MW: 64815 Da
• Target: This SLC23A1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human SLC23A1.
• UniProt ID: Q9UHI7
• NCBI: NP_005838.3, NP_689898.2
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: Solute carrier family 23 member 1, Na(+)/L-ascorbi
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

All lanes: Anti-SLC23A1 Antibody (N-term) at 1:1000-1:2000 dilution. Lane 1: Caki-1 whole cell lysate. Lane 2: HT-29 whole cell lysate. Lane 3: SW480 whole cell lysate.Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 65 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Immunohistochemical analysis of paraffin-embedded human small intestine tissue performed on the Leica BOND RXm. Tissue was fixed with formaldehyde at room temperature, antigen retrieval was by heat mediation with a EDTA buffer (pH9.0). Samples were incubated with primary antibody (1:500) for 1 hours at room temperature. A undiluted biotinylated CRF Anti-Polyvalent HRP Polymer antibody was used as the secondary Antibody.

SLC23A1 Antibody (N-term) western blot analysis in HepG2 (lane 1), NCI-H292 (lane 2) cell line lysates (35 ug/lane). This demonstrates the SLC23A1 antibody detected the SLC23A1 protein (arrow).