SLC1A1 Antibody

SKU: orb1258014

Description

SLC1A1 Antibody

Research Area

Neuroscience

Images & Validation

• Tested Applications: IF, WB
• Reactivity: Human, Mouse, Rat
• Application Notes:
  WB: 1:500 - 1:2000IF: 1:50 - 1:200

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 430-524 of human SLC1A1 (NP_004161.4).
• Target: SLC1A1
• Molecular Weight: Observed: 71kDa
• Purification: Affinity purification
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: PBS with 0.02% sodium azide, 50% glycerol, pH 7.3.
• Concentration: batch dependent
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

DCBXA, EAAC1, EAAT3, SCZD18, excitatory amino acid transporter 3, excitatory amino acid carrier 1, neuronal and epithelial glutamate transporter, sodium-dependent glutamate/aspartate transporter 3, solute carrier family 1 (neuronal/epithelial high affinity glutamate transporter, system Xag), member 1

Western blot analysis of extracts of various cell lines, using SLC1A1 antibody (orb1258014) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Immunofluorescence analysis of rat brain using SLC1A1 antibody (orb1258014) at dilution of 1:100. Blue: DAPI for nuclear staining.

Immunofluorescence analysis of mouse brain using SLC1A1 antibody (orb1258014) at dilution of 1:100. Blue: DAPI for nuclear staining.

Quick Database Links

Gene Symbol

SLC1A1

UniProt

P43005