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Item 1 of 6
Item 1 of 6
SLC16A3 Antibody (C-term)
Catalog Number: orb1937507
| Catalog Number | orb1937507 |
|---|---|
| Category | Antibodies |
| Description | Affinity Purified Rabbit Polyclonal Antibody (Pab) |
| Species/Host | Rabbit |
| Clonality | Polyclonal |
| Clone Number | RB31368 |
| Tested applications | IHC-P, WB |
| Reactivity | Human, Mouse, Rat |
| Isotype | Rabbit IgG |
| Antibody Type | Primary Antibody |
| Dilution range | WB: 1:2000, WB: 1:1000-1:2000, WB: 1:1000, WB: 1:1000, IHC-P-Leica: 1:500, IHC-P-Leica: 1:500 |
| Form/Appearance | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
| Conjugation | Unconjugated |
| MW | 49469 Da |
| Target | This SLC16A3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 433-462 amino acids from the C-terminal region of human SLC16A3. |
| UniProt ID | O15427 |
| NCBI | NP_004198.1, NP_001193881.1, NP_001035888.1, NP_001193880.1, NP_001193879.1, NP_001035887.1 |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles |
| Alternative names | Monocarboxylate transporter 4, MCT 4, Solute carri Read more... |
| Note | For research use only |
| Expiration Date | 12 months from date of receipt. |
All lanes: Anti-SLC16A3 Antibody (C-term) at 1:1000 dilution. Lane 1: Human skeletal muscle tissue lysate. Lane 2: Rat skeletal muscle tissue lysate.Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 49 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.
All lanes: Anti-SLC16A3 Antibody (C-term) at 1:1000-1:2000 dilution. Lane 1: NCI-H460 whole cell lysate. Lane 2: Hela whole cell lysate. Lane 3: HCT116 whole cell lysate. Lane 4: Neuro-2a whole cell lysate.Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 49 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.
All lanes: Anti-SLC16A3 Antibody (C-term) at 1:2000 dilution. Lane 1: Human skeletal muscle tissue lysate. Lane 2: C2C12 whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 49 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.
Immunohistochemical analysis of paraffin-embedded Human colon carcinoma tissue performed on the Leica BOND RXm. Tissue was fixed with formaldehyde at room temperature, antigen retrieval was by heat mediation with a EDTA buffer (pH9.0). Samples were incubated with primary antibody (1:500) for 1 hours at room temperature. A undiluted biotinylated CRF Anti-Polyvalent HRP Polymer antibody was used as the secondary Antibody.
Immunohistochemical analysis of paraffin-embedded Human skeletal muscle tissue performed on the Leica BOND RXm. Tissue was fixed with formaldehyde at room temperature, antigen retrieval was by heat mediation with a EDTA buffer (pH9.0). Samples were incubated with primary antibody (1:500) for 1 hours at room temperature. A undiluted biotinylated CRF Anti-Polyvalent HRP Polymer antibody was used as the secondary Antibody.
SLC16A3 Antibody (C-term) western blot analysis in NCI-H460 cell line lysates (35 ug/lane). This demonstrates the SLC16A3 antibody detected the SLC16A3 protein (arrow).
