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Catalog Number | orb1248402 |
---|---|
Category | Antibodies |
Description | SLAMF8 Antibody |
Species/Host | Goat |
Clonality | Polyclonal |
Tested applications | ELISA, IHC, WB |
Predicted Reactivity | Bovine, Mouse, Rat |
Reactivity | Human |
Immunogen | The immunogen for this antibody is: C-TLYHSRFLGRAQ |
Concentration | 500 ug/mL |
Dilution range | Peptide ELISA: antibody detection limit dilution 1:8000.Western Blot:In transfected HEK293 transiently expressing Human SLAMF8 (myc and DYKDDDDK tagged), a band of approx. 45kDa is observed. No bands are observed in mock-transfected HEK293 and the same band is observed using anti-myc antibody. Recommended concentration: 0.5-1ug/ml.Immunohistochemistry:Paraffin embedded Human Small Intestine. Recommended concentration: 5ug/ml. |
Form/Appearance | Liquid |
Conjugation | Unconjugated |
Target | SLAMF8 |
UniProt ID | Q9P0V8 |
NCBI | NP_064510.1 |
Storage | Aliquot and store at -20°C. Minimize freezing and thawing. |
Buffer/Preservatives | Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing. |
Alternative names | SLAMF8, SLAM family member 8, BLAME, FLJ20442, MGC Read more... |
Note | For research use only |
Application notes | Peptide ELISA: antibody detection limit dilution 1:8000.Western Blot:In transfected HEK293 transiently expressing Human SLAMF8 (myc and DYKDDDDK tagged), a band of approx. 45kDa is observed. No bands are observed in mock-transfected HEK293 and the same band is observed using anti-myc antibody. Recommended concentration: 0.5-1ug/ml.Immunohistochemistry:Paraffin embedded Human Small Intestine. Recommended concentration: 5ug/ml. |
Expiration Date | 12 months from date of receipt. |
HEK293 lysate (10 ug protein in RIPA buffer) overexpressing Human SLAMF8 with C-terminal MYC tag probed with orb1248402 (1 ug/ml) in Lane A and probed with anti-MYC Tag (1/1000) in lane C. Mock-transfected HEK293 probed with orb1248402 (1 ug/ml) in Lane B.
orb1248402 (5 ug/ml) staining of paraffin embedded Human Small Intestine. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
ELISA, IHC, WB | |
Bovine, Human, Mouse, Rat | |
Goat | |
Polyclonal | |
Unconjugated |
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