Skepinone-L

SKU: orb1301048

Description

Skepinone-L (CBS3830) is a potent and selective inhibitor of the p38 MAP kinase. This small molecule is utilized in biochemical and cellular research to investigate inflammatory pathways and stress responses, with applications in both in vitro and in vivo experimental models.

Research Area

Cell Biology, Signal Transduction

Key Properties

• CAS Number: 1221485-83-1
• MW: 425.42
• Purity: >99.99% (May vary between batches)
• Formula: C₂₄H₂₁F₂NO₄
• SMILES: OC[C@@H](O)COc1ccc2CCc3cc(Nc4ccc(F)cc4F)ccc3C(=O)c2c1
• Target: p38 MAPK,Autophagy
• Solubility: DMSO:50 mg/mL (117.53 mM);H2O:< 1 mg/mL (insoluble or slightly soluble);Ethanol:79 mg/mL (185.7 mM);10% DMSO+40% PEG300+5% Tween 80+45% Saline:2 mg/mL (4.7 mM)

Bioactivity

• Target IC50:
  p38α:5 nM
• In Vivo:
  In vivo, skepinone-L inhibits Gal/ LPS-induced TNF-α release by 77%.
• In Vitro:
  Skepinone-L shows concentration-dependent inhibition of HSP27 (Ser82) phosphorylation through the p38 MAPK pathway with a cellular IC50 of approximately 25 nM, and also reduces concentrations of TNF-α, IL-1β and IL-10, regulated by p38 MAPK, with IC50 ranging from 30 to 50 nM . Skepinone-L (1 μM) abrogates the phosphorylation of platelet p38 MAPK substrate Hsp27 activated by stimulation with CRP, thrombin or thromboxane A2 analogue U-46619, and impairs platelet secretion and aggregation.
• Cell Research:
  Concentrations: 1 nM - 10 μM, dissolved in DMSO

Storage & Handling

• Storage: Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

CBS3830, CBS-3830, CBS 3830, Autophagy, Inhibitor, inhibit, Skepinone-L, SkepinoneL, Skepinone L, p38MAPK, p38α, p38 MAPK

Compound Identifiers

PubChem CID

45279963