Sirtuin1 (SIRT1) Antibody

SKU: orb1410127

Description

Mouse monoclonal antibody to Sirtuin1 (SIRT1)

Images & Validation

• Tested Applications: ELISA, FC, IF, WB
• Reactivity: Human

Key Properties

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG1
• Clone No.: PCRP-SIRT1-1E11
• Immunogen: Recombinant full-length human SIRT1protein
• Target: SIRT1
• Source/Expression System: Bioreactor
• Molecular Weight: 120kDa
• Purification: Protein G
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% rAlbumin & 0.05% azide. Also available WITHOUT rAlbumin & azide at 1.0mg/ml.
• Disclaimer: For research use only

Alternative Names

75SirT1; hSIR2; hSIRT1; NAD dependent deacetylase sirtuin 1; NAD-dependent deacetylase sirtuin-1; Regulatory protein SIR2 homolog 1; SIR2 like protein 1; SIR2, S.cerevisiae, homolog-like 1; SIR2ALPHA; SIR2L1; SirtT1 75 kDa fragment; Sirtuin (silent mating type information regulation 2 homolog) 1 (S. cerevisiae); Sirtuin type 1

Analysis of Protein Array containing more than 19000 full-length human proteins using SIRT1-Monospecific Mouse Monoclonal Antibody (PCRP-SIRT1-1E11). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.

Flow cytometric analysis of PFA-fixed HeLa cells. SIRT1 Mouse Monoclonal Antibody (PCRP-SIRT1-1E11) followed by goat anti-mouse IgG-CF488 (blue); isotype control (red).

Immunofluorescence Analysis of PFA-fixed HeLa cells stained using SIRT1 Mouse Monoclonal Antibody (PCRP-SIRT1-1E11) followed by goat anti-mouse IgG-CF488 (blue); isotype control (red).

SDS-PAGE Analysis of Purified SIRT1 Mouse Monoclonal Antibody (PCRP-SIRT1-1E11). Confirmation of Purity and Integrity of Antibody.

Quick Database Links

Gene Symbol

SIRT1

UniProt

Q96EB6