SIRT7 Antibody (C-term)

SKU: orb1930158

Description

Purified Rabbit Polyclonal Antibody (Pab)

Research Area

Epigenetics & Chromatin

Images & Validation

• Tested Applications: IHC-P, WB
• Dilution Range: WB - 1:2000, IHC-P - 1:25
• Reactivity: Human
• Predicted Reactivity: Mouse, Rat

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Clone No.: BTD3195
• Immunogen: This SIRT7 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 331-360 amino acids from the C-terminal region of human SIRT7. Antigen Region: 331-360 aa.
• Target: SIRT7 {ECO:0000303|PubMed:22722849, ECO:0000312|HGNC:HGNC:14935}
• Molecular Weight: 44898 Da
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

NAD-dependent protein deacetylase sirtuin-7, 351-, Regulatory protein SIR2 homolog 7, SIR2-like protein 7, SIRT7, SIR2L7

Staining SIRT7 in human esophagus tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0.5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.

Western blot analysis of SIRT7 (arrow) using SIRT7 Antibody (C-term). HEK293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the SIRT7 gene (Lane 2).

Anti-SIRT7 Antibody (C-term) at 1:2000 dilution + H-4-II-E whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 45 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Quick Database Links

Gene Symbol

SIRT7 {ECO:0000303|PubMed:22722849, ECO:0000312|HGNC:HGNC:14935}

UniProt

Q9NRC8

RefSeq (Protein):NP_057622.1