SIRT7 Antibody (C-term)

• Catalog Number: orb1930158
• Category: Antibodies
• Description: SIRT7 Antibody (C-term)
• Clonality: Polyclonal
• Species/Host: Rabbit
• Isotype: Rabbit IgG
• Conjugation: Unconjugated
• Reactivity: Human, Mouse, Rat
• Predicted Reactivity: Bovine
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• UniProt ID: Q9NRC8
• MW: 44898 Da
• Tested applications: IHC-P, WB
• Dilution range: IHC-P - 1:100-500, WB - 1:1000
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Research Area: Cancer Biology, Cardiovascular Research, Metabolis
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Staining SIRT7 in human esophagus tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0.5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.

Western blot analysis of SIRT7 (arrow) using SIRT7 Antibody (C-term). HEK293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the SIRT7 gene (Lane 2).

Anti-SIRT7 Antibody (C-term) at 1:2000 dilution + H-4-II-E whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 45 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.