SIRT1/Sirtuin 1 Antibody

• Catalog Number: orb1538354
• Category: Antibodies
• Description: SIRT1/Sirtuin 1 Antibody
• Target: SIRT1 / Sirtuin 1
• Clonality: Monoclonal
• Species/Host: Mouse
• Isotype: IgG1,k
• Conjugation: Unconjugated
• Reactivity: Human
• Concentration: 0.5 mg/ml
• Buffer/Preservatives: PBS, pH 7.4. PBS, pH 7.4
• Purification: Purified from ascites by Protein A
• Immunogen: SIRT1 (AAH12499, 456 aa ~ 555 aa) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
• Tested applications: ELISA, IF, IHC, IHC-P, WB
• Dilution range: ELISA, IF (10 µg/ml), IHC, IHC-P (5 µg/ml), WB
• Application notes: Further information: Western Blot (Cell lysate) - positive control Hela NE. Immunofluorescence (10 ug/ml) - positive control HeLa cells. Sandwich ELISA (Recombinant protein).
• Clone Number: 7B7
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: SIRT1, SIR2-like protein 1, SIR2alpha, SIR2L1, Sir
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Immunofluorescence of monoclonal antibody to SIRT1 on HeLa cell (antibody concentration 10 ug/ml).

SIRT1 monoclonal antibody, clone 7B7 Western blot of SIRT1 expression in HeLa NE.

Detection limit for recombinant GST tagged SIRT1 is approximately 0.1 ng/ml as a capture antibody.