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Description
Research Area
Cell Biology
Images & Validation
−Item 1 of 4
| Tested Applications | FC, ICC, IHC, WB |
|---|---|
| Dilution Range | WB: 1/500 - 1/2000; IHC: 1/200 - 1/1000; IF/ICC: 1/200 - 1/1000; FCM: 1/200 - 1/400; ELISA: 1/10000 |
| Reactivity | Human, Monkey, Mouse, Rabbit, Rat |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | Mouse IgG1 |
| Clone No. | B3H5 |
| Immunogen | Purified recombinant fragment of human SIRT1 expressed in E. Coli. |
| Target | SIRT1 |
| Molecular Weight | 120kDa |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Buffer/Preservatives | Purified antibody in PBS with 0.05% sodium azide. |
| Concentration | Approximately 1mg/ml (varies from batch to batch). Please inquire for precise concentration. |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−SIR2L1; SIRT1
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Quality Guarantee
Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our Terms & Conditions and Buying Guide. Contact us at [email protected].
Flow cytometric analysis of K562 cells using SIRT1 antibody
Immunofluorescence analysis of NTERA-2 cells using SIRT1 antibody
Western blot analysis of MCF-7 (Lane1), Jurkat (Lane2), Hela (Lane3), HEK293 (Lane4) and A549 (Lane5) cell lysate using SIRT1 ant
IHC analysis of orb69300.
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Product Information
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC
Immunohistochemistry
FC
Flow Cytometry
ICC
Immunocytochemistry
SIRT1 Antibody (orb69300)
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