SIRT1 Antibody

SKU: orb1927235

Description

Purified Mouse Monoclonal Antibody (Mab)

Research Area

Cell Biology

Images & Validation

• Tested Applications: WB
• Dilution Range: WB - 1:1000
• Reactivity: Human, Mouse

Key Properties

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG3,k
• Clone No.: B3201EV7X5X3
• Immunogen: This antibody is generated from a mice immunized with a recombinant protein between 1-180 amino acids from human.
• Target: SIRT1 {ECO:0000303|PubMed:12535671, ECO:0000312|HGNC:HGNC:14929}
• Molecular Weight: 81681 Da
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Form/Appearance: Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

NAD-dependent protein deacetylase sirtuin-1, hSIRT1, 351-, Regulatory protein SIR2 homolog 1, SIR2-like protein 1, hSIR2, SirtT1 75 kDa fragment, 75SirT1, SIRT1, SIR2L1

Western blot analysis of lysates from A549, 293 cell line (from left to right), using RPS6KB2 Antibody. Diluted at 1:1000 at each lane. A goat anti-mouse IgG H&L (HRP) at 1: 3000 dilution was used as the secondary antibody. Lysates at 35μg per lane.

All lanes: Anti-SIRT1 Antibody at 1:1000 dilution. Lane 1: Jurkat whole cell lysate. Lane 2: F9 whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 82 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Quick Database Links

Gene Symbol

SIRT1 {ECO:0000303|PubMed:12535671, ECO:0000312|HGNC:HGNC:14929}

UniProt

Q96EB6