SIRT1 Antibody

• Catalog Number: orb1927235
• Category: Antibodies
• Description: SIRT1 Antibody
• Clonality: Monoclonal
• Species/Host: Mouse
• Isotype: IgG3,k
• Conjugation: Unconjugated
• Reactivity: Human, Mouse
• Form/Appearance: Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Euglobin precipitation followed by dialysis against PBS.
• UniProt ID: Q96EB6
• MW: 81681 Da
• Tested applications: WB
• Dilution range: WB - 1:1000
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: SIR2L1
• Research Area: Cell Biology, Infectious Disease & Virology, Metab
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western blot analysis of lysates from A549, 293 cell line (from left to right), using RPS6KB2 Antibody. Diluted at 1:1000 at each lane. A goat anti-mouse IgG H&L (HRP) at 1: 3000 dilution was used as the secondary antibody. Lysates at 35μg per lane.

All lanes: Anti-SIRT1 Antibody at 1:1000 dilution. Lane 1: Jurkat whole cell lysate. Lane 2: F9 whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 82 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.