SIRT1 Antibody, KO Validated

• Catalog Number: orb1274544
• Category: Antibodies
• Description: SIRT1 Antibody, KO Validated
• Target: SIRT1
• Clonality: Polyclonal
• Isotype: IgG
• Conjugation: Unconjugated
• Reactivity: Human, Mouse, Rat
• Form/Appearance: Liquid
• Concentration: batch dependent
• Buffer/Preservatives: Liquid
• Purification: Affinity purification
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 448-747 of human SIRT1 (NP_036370.2).
• UniProt ID: Q96EB6
• MW: Observed: 140kDa
• Tested applications: IF, IHC, IP, WB
• Application notes: WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200IP: 1:50 - 1:100
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: SIRT1 Antibody: SIR2L1, SIR2L1, NAD-dependent prot
• Research Area: Cell Biology
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western blot analysis of extracts of various cell lines, using SIRT1 antibody (orb1274544) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Western blot analysis of extracts from normal (control) and SIRT1 knockout (KO) HeLa cells, using SIRT1 antibody (orb1274544) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Immunohistochemistry of paraffin-embedded human mammary cancer using SIRT1 antibody (orb1274544) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded human tonsil using SIRT1 antibody (orb1274544) at dilution of 1:100 (40x lens).

Immunofluorescence analysis of HeLa cells using SIRT1 antibody (orb1274544) at dilution of 1:100. Blue: DAPI for nuclear staining.

Immunoprecipitation analysis of 200 ug extracts of Jurkat cells, using 3 ug SIRT1 antibody (orb1274544). Western blot was performed from the immunoprecipitate using SIRT1 antibody (orb1274544) at a dilution of 1:1000.