SIRP Alpha Rabbit Polyclonal Antibody

SKU: orb4840

Description

SIRP Alpha Rabbit Polyclonal Antibody

Research Area

Immunology & Inflammation

Images & Validation

• Tested Applications: FC, WB
• Dilution Range: WB=1:500-2000, Flow-Cyt=1μg /test
• Reactivity: Human, Mouse, Rat
• Predicted Reactivity: Bovine, Canine, Equine, Porcine

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human CD172a (151-250/504aa)
• Target: SIRPA
• Molecular Weight: 56 kDa
• Purification: Affinity purified by Protein A
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

BIT; CD172A; MFR; MYD-1; MYD1; P84; PTPNS1; SHPS1; SIRP; Idd13.2; SHP-1; SHPS-1; SHPS1_HUMAN; SIRPA; SHP substrate 1; Brain Ig-like molecule with tyrosine-based activation motifs (Bit); CD172 antigen-like family member A; Inhibitory receptor SHPS-1; Macrophage fusion receptor; MyD-1 antigen; Signal-regulatory protein alpha-1 (Sirp-alpha-1); Signal-regulatory protein alpha-2 (Sirp-alpha-2); Signal-regulatory protein alpha-3 (Sirp-alpha-3); SHPS1_MOUSE; Signal-regulatory protein alpha-1 (Sirp-alpha-1 | mSIRP-alpha1); SHPS1_RAT; Macrophage membrane protein MFP150;

Blank control (blue): U937 (fixed with 2% paraformaldehyde (10 min)). Primary Antibody: Rabbit Anti-SIRP antibody (orb4840), dilution: 1 µg in 100 µl 1X PBS containing 0.5% BSA, Isotype Control Antibody: Rabbit IgG (orange), used under the same conditions, Secondary Antibody: Goat anti-rabbit IgG-PE (white blue), dilution: 1:200 in 1X PBS containing 0.5% BSA.

Sample: Lane 1: Mouse Brain Lysates, Lane 2: Mouse Cerebellum Lysates, Lane 3: Mouse Spleen Lysates, Lane 4: Mouse Placenta Lysates, Lane 5: Rat Brain Lysates, Lane 6: Rat Cerebellum Lysates, Primary: Anti-SIRP Alpha (orb4840) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 56kDa, Observed band size: 56kDa.

Quick Database Links

Gene Symbol

SIRPA

UniProt

P78324