SIRL-1, Human, mAb 1A5 Antibody

SKU: orb1108831

Description

Mouse monoclonal antibody to SIRL-1

Research Area

Immunology & Inflammation

Images & Validation

• Tested Applications: FC, Functional Studies, IP, WB
• Dilution Range: For immuno precipitation, flow cytometry and Western blotting, dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50.
• Reactivity: Human
• Application Notes:
  W: A non-reduced sample treatment and SDS-Page was used. The band sizes are 45 kDa and fainter band of 37 kDa in transfected cells and 37 kDa in granulocytes (Ref.1). FS: Antibody 1A5 showed inhibition of FcεRI-mediated degranulation by RBL-2H3 cells transfected with SIRL-1. The percentage of inhibition of degranulation by SIRL-1 was calculated by (OD405 TNP stimulation €" OD405 TNP with SIRL-1 crosslinking)/(OD405 TNP stimulation €" OD405 spontaneous release) x 100.

Key Properties

• Clonality: Monoclonal
• Isotype: Mouse IgG1
• Immunogen: Purified SIRL-1-hIg fusion protein in PBS
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: 1 ml (100 µg/ml) 0.2 µm filtered antibody solution in PBS, containing 0.1% bovine serum albumin.
• Buffer/Preservatives: 0.2 ml (100 µg/ml) 0.2 µm filtered antibody solution in PBS, containing 0.1% bovine serum albumin.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

V-set and transmembrane domain-containing protein 1, Signal inhibitory receptor on leukocytes-1

Quick Database Links

UniProt

Q6UX27