SIN1 Antibody

• Catalog Number: orb248120
• Category: Antibodies
• Description: mTOR, or Mammalian target of rapamycin, a large protein kinase, is a regulator of cell growth and survival via its mTORC1 (mTOR + GBL + Raptor) and mTORC2 (mTOR + GBL + SIN1 + Rictor) complexes. SIN1 is an essential component of mTORC2, which activates Akt by phosphorylating Ser473. Phosphorylation of SIN1 at Thr86/398 causes its dissociation from the mTORC2 complex, inhibiting mTORC2s kinase activity.
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: WB
• Reactivity: Canine, Human, Mouse, Rat
• Isotype: Rabbit IgG
• Immunogen: A recombinant protein fragment from the C-terminal region of human SIN1 was used as the immunogen for this antibody.
• Dilution range: Western blot: 1-3ug/ml
• Purity: Protein A purified antibody
• Conjugation: Unconjugated
• Formula: 0.5 mg/ml with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
• Hazard Information: This SIN1 antibody is available for research use only.
• Entrez: 79109
• Storage: Aliquot and store frozen at -20°C or colder to avoid repeated freeze-thaw cycles.
• Buffer/Preservatives: 1X PBS, pH 7.4
• Note: For research use only
• Application notes: Provided assay concentrations are suggestions only, SIN1 antibody titration may be required for optimal results.
• Expiration Date: 12 months from date of receipt.

Western blot analysis of human samples using SIN1 antibody (primary antibody dilution at: at 1 ug/ml)