Simvastatin

SKU: orb1310385

Description

Simvastatin

Research Area

Cell Biology, Metabolism Research

Key Properties

• CAS Number: 79902-63-9
• MW: 418.57
• Purity: 99.95%
• Formula: C₂₅H₃₈O₅
• SMILES: CCC(C)(C)C(=O)O[C@H]1C[C@@H](C)C=C2C=C[C@H](C)[C@H](CC[C@@H]3C[C@@H](O)CC(=O)O3)[C@@H]12
• Target: HMG-CoA Reductase,Ferroptosis,Mitophagy,Apoptosis,Autophagy
• Solubility: Ethanol:100 mg/mL (238.91 mM);10% DMSO+40% PEG300+5% Tween 80+45% Saline:5 mg/mL (11.95 mM);DMSO:50 mg/mL (119.45 mM)

Bioactivity

• Target IC50:
  L6 cells:229 nM|Hepatocyte cells:1.3 nM|MEF cells:36.7 μM|HEK293 cells:11 μM|Hs 68 cells:26.4 μM|A549 cells:16.3 μM|HMG-CoA reductase:0.1-0.2 nM (Ki)
• In Vivo:
  METHODS: To study in vivo activity, Simvastatin (60 mg/kg, aqueous 2% DMSO+30% PEG 400+5% Tween 80) was administered by gavage to C57BL/6J mice once daily for six weeks on a CF diet. RESULTS: Simvastatin treatment reduced serum cholesterol levels by 18%, and retinal cholesterol and lipoprotein cholesterol levels by 24% and 21%, respectively. METHODS: To assay antitumor activity in vivo, Simvastatin (5-50 mg/kg in methylcellulose) was administered to BALB/c nu/nu mice by gavage once daily for three days. Subsequently, colorectal cancer cells COLO205 were subcutaneously inoculated into the right side of the mice. RESULTS: Simvastatin inhibited tumor growth in a xenograft mouse model by inducing tumor cell apoptosis and inhibiting tumor angiogenesis.
• In Vitro:
  METHODS: BCa cells 5637, EJ and T24 were treated with Simvastatin (0.5-40 µM) for 48 h. Cell viability was measured by MTT assay. RESULTS: Simvastatin significantly inhibited the survival of the three BCa cells in a dose-dependent manner. METHODS: Human fibroblast SAEC and four tumor cells MCF7, HepG2, NCH, NCI were treated with Simvastatin (20 µM) for 72 h. Apoptosis was detected by TUNEL. RESULTS: Simvastatin induced apoptosis in different types of human tumor cells, but not in SAEC cells.

Storage & Handling

• Storage: Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

oral activity, MK733, MK-733, MK 733, MK0733, MK-0733, Mitophagy, MK 0733, Mitochondrial Autophagy, inhibit, Inhibitor, HepG2, HMGCoA Reductase, HMG-CoA Reductase (HMGCR), HMGCoAReductase, HMG-CoA reductase, HMGCR, Ferroptosis, Huh7, cholesterol synthesis, Autophagy, anticancer, anti-proliferation activity, Apoptosis, Simvastatin

Compound Identifiers

PubChem CID

54454